Introducing Immunofluorescence Microscopy of Tetrahymena Cells in Cell Biology Lab

Abstract

Immunofluorescence (IF) microscopy is an essential laboratory technique within the field of biological sciences. This technique offers high sensitivity to observe biological samples so that scientists are able to visualize cellular and sub-cellular structures that may be too difficult to be observed with a traditional light microscope. It works by two main procedures, IF staining that is to tag the sample with a single antibody linked to a fluorophore, and IF microscopy that is to illuminate the sample with blue or UV light. Mastering this technique enables students to gain a greater understanding of biological systems by further enhancing optical resolution. The primary goals of this project are to (1) aid students in gaining a basic understanding of the principle of IF staining and IF microscopy by allowing hands-on experience in labeling and imaging biological samples, (2) introduce a dedicated module on IF microscopy to cell biology students and to establish this module as an official UNG cell biology laboratory procedure, and (3) emphasize the importance of this technique to students’ future science career and to encourage students with the confidence to navigate and excel in advanced laboratory settings. This is a project that will be implemented in Fall 2023. The outcomes of this project include (1) developing a laboratory procedure on IF microscopy and student handouts for the UNG cell biology course, (2) developing student assessments to test students’ pre-existing knowledge and the application of IF microscopy, and (3) presenting our work to the public audience.